Autotrophic (C1-gas) versus heterotrophic (fructose) accumulation of acetic acid and ethanol in Clostridium aceticum

The influence of the carbon source on metabolism and growth Clostridium aceticum was investigated, supplying either CO or fructose as sole source. acid solvent production patterns were determined under autotrophic heterotrophic conditions, elucidating effect pH substrate's bioconversion pattern. highest maximum specific rate observed with CO, organism's optimal reaching 0.052 h−1 an acetic concentration 18 g·L−1. 4.4 g·L−1 ethanol also possible, after medium acidification, during bioconversion. Conversely, formic inhibition fermentation conditions. In latter experiments, it not possible to stimulate when growing C. fructose, despite applying same acidification strategy showing selective (autotrophic vs heterotrophic) metabolic pattern solventogenesis.